Immunomodulatory role of Clarithromycin in Acinetobacter baumannii infection via Neutrophil Extracellular Traps formation.

ΤίτλοςImmunomodulatory role of Clarithromycin in Acinetobacter baumannii infection via Neutrophil Extracellular Traps formation.
Publication TypeJournal Article
Year of Publication2015
AuthorsKonstantinidis T, Kambas K, Mitsios A, Panopoulou M, Tsironidou V, Dellaporta E, Kouklakis G, Arampatzioglou A, Angelidou I, Mitroulis I, Skendros P, Ritis K
JournalAntimicrobial agents and chemotherapy
Date Published2015 Dec 7
ISSN1098-6596
Abstract

Macrolide antibiotics have been shown to act as immunomodulatory molecules in various immune cells. However, their effect on neutrophils has not been extensively investigated. In this study we investigated the role of macrolide antibiotics in the generation of NETs.By assessing ex vivo and in vivo NET formation we demonstrated that clarithromycin is able to induce NET generation both in vitro and in vivo. Clarithromycin utilizes autophagy in order to form NETs and these NETs are decorated with antimicrobial peptide LL-37. Clarithromycin-induced NETs are able to inhibit Acinetobacter baumannii growth and biofilm formation in a LL-37-dependent manner. Additionally, LL-37 antimicrobial function depends on NET-scaffold integrity.Collectively, these data expand the knowledge on the immunomodulatory role of macrolide antibiotics via the generation of LL-37-bearing NETs, which demonstrate LL-37-dependent antimicrobial activity and biofilm inhibition against A. baumannii.

DOI10.1128/AAC.02063-15
Alternate JournalAntimicrob. Agents Chemother.